BrdU-labelling in 3rd larvae of Drosophila

From: Kai Herrmann (kherrma@uni-muenster.de)
Date: Tue Jan 18 2000 - 06:00:34 EST


I'm trying to label imaginal cells in larvae using a BrdU-antibody 
method, by feeding the larvae a BrdU-yeast mixture (1mg/ml yeast) 
over 2-4 hrs.
However, the results are not as satisfactory as I'd wish, as only 
very few cells seem to have incorporated the BrdU.
Has anyone any suggestions for the above mentioned method and can 
give me some advice on how to improve its outcome?
Many thanks in advance,

Kai Herrmann

AG Prof. Janning
Univ. Münster
Germany
e-mail: <kherrma@uni-muenster.de>



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