I'm trying to label imaginal cells in larvae using a BrdU-antibody method, by feeding the larvae a BrdU-yeast mixture (1mg/ml yeast) over 2-4 hrs. However, the results are not as satisfactory as I'd wish, as only very few cells seem to have incorporated the BrdU. Has anyone any suggestions for the above mentioned method and can give me some advice on how to improve its outcome? Many thanks in advance, Kai Herrmann AG Prof. Janning Univ. Münster Germany e-mail: <kherrma@uni-muenster.de>
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