Dear Flow People, I would like to systematically study the effects of chronic (weeks) of elevated ambient temperature (mid-high 30's oC) on the mouse immune system. Can anyone provide step-by-step protocols (NOT Becton-Dickinson's, which I have) and, if possible, citations, for the following flow procedures, to be carried out on mouse splenocytes or plasma? (I already have info on CD4, CD8, Anti-LPS IgG) The information, with thanks, will be properly cited in all subsequent publications. * NK cell numbers and NK activation * Lymphocyte activation * Phagocytosis (fluorescent beads) * Proliferation of any cell type * Reactive oxygen species * PKC activity * Phospholipase activity * Intracellular pH\ * Membrane potential Thank you, Steve Gaffin ************************************************************ ************************************************************ ** Stephen L. Gaffin, Ph.D. ** ** Research Physiologist ** ** U.S. Army Research Institute for Environmental Medicine ** ** Natick MA 01760-5007 ** ** Tel. 508-233-4867 Fax 508-233-4869 ** ** email stephen.gaffin@na.amedd.army.mil ** ************************************************************ ************************************************************
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