Royal Microscopical Society

Flow cytometry courses

Sheffield Medical School

13 – 17 September 1999

The Complete Programme can be found at http://www.uwcm.ac.uk/uwcm/hg/hoy.

Course organisers

Michael G. Ormerod, Reigate. mailto:Michael_Ormerod@compuserve.com

John Lawry, Institute for Cancer Studies, Sheffield. mailto:J.Lawry@Sheffield.ac.uk

About the Courses

The course has been constructed as a set of modules. The student can elect to attend the Course from between five days to a single day, depending on the Modules selected.

Each Module will consist of lectures interspersed with sessions in the laboratory. We anticipate that instruments from three manufacturers will be available for practical work.

For Module 1, no prior knowledge of flow cytometry is assumed. It will give the student a firm grounding in the basics of flow cytometry. There is also a strong emphasis on practical application and four practical sessions are included.

The later Modules assume that the student has some basic knowledge of flow cytometry (possibly obtained by taking Module 1).

Module 2, Clinical Applications, will include practical sessions and lectures on immunophenotyping leukaemias, immune monitoring, applications to transplantation and blood transfusion and quality control.

Module 3 will include a study of the DNA histogram and the cell cycle together with sessions on measuring cell cycle kinetics in cultures and in tumours in vivo, using labelling with bromodeoxyuridine, and the measurement of cell cycle related proteins.

Module 4 will include sessions on apoptosis (including practical work), drug resistance, cell cycle kinetics and microbiology.

During the cell sorting module (Module 5), we expect to demonstrate cell sorting on four different instruments.

Laser Scanning Cytometry (Module 6) introduces a relatively new technology that is complementary to Flow Cytometry. An instrument will be available for practical work.

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Structure of the Courses

Students may take the full five day course or may opt to take a three day course- either the Basic or the Advanced Course.

Full Course: 13-17 September. Module 1, Module 2 or 3, Module 4 plus Module 5 or 6.

Basic Course: 13-15 September. Module 1 plus Module 2 or 3.

Advanced Course: 15-17 September. Module 2 or 3, Module 4, Module 4 or 5.

Students may also take individual modules.

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The Modules

Module 1 Basics of flow cytometry. Two days; 13th - 14th.

Module 2 Clinical applications. One day; 15th.

Module 3 Measurement of tumour cell kinetics. One day; 15th.