TUNEL Assay

From: Girakhoo, Soheila (Girakhoo@cber.fda.gov)
Date: Tue Dec 28 1999 - 15:26:29 EST

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Dear Flowers,

I have a question about the Tunel assay using flow cytometry. I treated my
cells ( adherent cell line) with agents, Actinomysin D, Ceramide,
Campothecin, before staining with dUTP-FITC (kit from boehringer mannhein)
to detect apoptosis.What does mean when the label sample(negative sample) is
higher( histogram peak) than positive sample (TdT sample).
I would appreciate any help anyone can give.

Thanks,

Soheila

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