Greetings once again, first, Happy Holidays everyone! I hope I get all the replies to this message before y2k bug chews up all the wiring in our computers :) issue #1 I have recently been working with a PI who studies epithelial cells. During acquisition, my instrument was calibrated using BD calibrite beads, 2 color. I noticed that using ssc @ 1.00 and fsc @ 2.00 a lot of those cells would still not "fit" in the panel. I decreased fsc to 1.00 and then decided to change fsc and ssc modes from log to lin. The question is, by doing that, am I acquiring more events or less? Is the coverage of ssc vs. fsc broader in lin or log? If there's a FAQ on this I would love to read it. issue#2 (a little off topic..) The PI has some problems with epithelial cells' clumping and adhering. He was told to try trypsin to get them off the plastic tubes, but he is afraid that might affect his protein of interest which is expressed on the surface. Another thing he tried was to soak his cells in PBS without calcium, but he thinks they tend to clump that way and it's hard to obtain cell counts. What is the best way to prepare epithelial cells for flow? Thanks in advance for any responses. ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Thousands of Stores. Millions of Products. All in one place. Yahoo! Shopping: http://shopping.yahoo.com
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:22 EST