Derek We are doing DNA analysis on animal tissue. This tissue contains many different sized G2 cells and aggregates in the polyploid region. We notice that there is about 10% of the nuclei that are located in the polyploid region. A program from Verity software-- MODFIT LT2.0 uses aggregates in this region to subtract the amount of doublets from the G2 region. We have compared the width vs area with the aggregate model and find the fit for DNA analysis is superior with this aggregate model with animal tissues. I was a little surprised with the data as we previously have always done DNA analysis using this with vs area model of discrimination. good luck Question : why are there so many cells in this polyploid area--is this a cycling population, aggregates or ??? Robert M. Zucker, Ph.D U.S. Environmental Protection Agency National Health and Environmental Effects Research Laboratory 2525 NC Highway 54, MD 72 Research Triangle Park, North Carolina, 27709 tel: 919-541-1585 fax 919-541-4017 e-mail: zucker.robert@epa.gov
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