Dear Neo Sim Yee, Possibly the most suitable inactivation process is gamma irradiation. A company called BTF Pty Ltd in Australia (fx: (02) 9948 9112; email - btf@acay.com.au; contact Dr. Graham Vesey) prepare flow sorted- gamma irradiated cysts/oocysts called EasySeed. The EasySeed should be ideal for your purposes as it contains a stable, inactivated population of approx. 100 cysts/oocysts (cv>3%). Ensuring accuracy in spike/seed experiments is one of the most crucial and labour intensive areas in G/C work. EasySeed simplifies this. Dr. Vesey also performed the definitive work using flow cytrometry and G/C. Regards Andrew Campbell Dynal AS Norway
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