The frozen target cells are shipped in 5 aliquots. Their viability is good (>90%) for up to three months (>95% after arrival). You should contact the people at ORPEGEN (www.orpegen.com) ahofmann@orpegen.com or whirt@orpegen.com for extra vials. The non-MHC-restricted T cells (CD3+/CD16+56+) are below 5% or 150/µl. They may be increased in chronical viral infections (eg. CMV) and tumor patients (eg. breast cancer). T-B cells are artifacts or true cell communication events (culture). They are below 0.1% in normal samples from peripheral blood (whole blood, FACS lysing solution, wash method). Thomas Nebe Dr.med. C. Thomas Nebe Universitaetsklinikum Mannheim Zentrallabor Theodor-Kutzer-Ufer 1-3 D-68167 Mannheim Tel. +49 621 383-3485 FAX +49 621 383-73 3485 +49 621 383-3819 e-mail: thomas.nebe@ikc.ma.uni-heidelberg.de Bitte besuchen Sie unsere informativen Webseiten unter http://www.ma.uni-heidelberg.de/inst/ikc/
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