That's right, it goes on even if drops are not correct and sorting the wrong cells, but it has nothing to do with the plates because sorting pulses are applied on the stream and plates are constantly charged. Sasha. On Tue, 07 Dec 1999 17:43:27 +0100 Ann Atzberger <Ann.Atzberger@EMBL-Heidelberg.de> wrote: > > O.K Sasha, > > but if your drop delay isn't right, it will still going to go on counting > left and right even though it may not be sorting any cells. It will also go > on counting if you turn off the deflection plates by mistake, which made me > come to the conclusion that it wasn't counting deflection pulses. > > Ann > At 15:52 07.12.99 +0000, you wrote: > >Hi Ann, > > > >I just want to express slight disagreement on sorting counters. > >On BD machines SORT L and R counters are actually counting deflection > >pulses, meaning that number given is: > > > >events that fit gates - (analysis aborts + coincidence aborts). > > > >Analysis aborts can be caused in three ways. Most important type is > >threshold abort - if second event occurs within the dead time of the > >previous one or if the first pulse exceeds its own dead time interval > >(pulse fails to drop bellow threshold by its end). > >On the other hand coincidence aborts would depend on sort mode, drop > >envelope size and drops/1cell ratio (drive frequency/event rate). > > > >Cheers, > >Sasha. > > > >On Mon, 06 Dec 1999 12:02:07 +0100 Ann Atzberger > ><Ann.Atzberger@EMBL-Heidelberg.de> wrote: > > > >> > >> > >> Hi Chris, > >> > >> in our case the cells were actually lysing as they weren't compatible with > >> the medium being used. > >> I'd also do a drop delay check. The cell number on the instrument counts > >> all the cells that fit in the sort gates but that does not mean it has > >> actually sorted it. > >> > >> regards > >> Ann > >> > >> At 13:35 02.12.99 -0800, you wrote: > >> > > >> >Hello gang, > >> >My question deals with our FacStar. I am getting reports of counts that > are > >> >about 1/2 of the cell number the instrument says were recovered. I am > >> >wondering if anyone has seen this or what to do to fix this problem. Cell > >> >count is very important in many of our studies. > >> >Thanks in advance and Happy Holidays, > >> > > >> >chris > >> > > >> > > >> > > >> > > >> > > >> >Chris Lena > >> >Laboratory Technician > >> >La Jolla Institute For Allergy and Immunology > >> >phone:(858)678-4536 > >> >fax:(858)678-4595 > >> >cali@liai.org > >> >http://www.liai.org > >> > > >> > _ _ > >> > (_)-(_) > >> > \"/ > >> > =V= > >> > > >> > > >> > > >> > > >> > > >> > > > >---------------------- > >Dr Sasha Sreckovic > >Dept Path & Micro > >University of Bristol > >University Walk > >Bristol, BS8 1TD, UK > >Sasa.Sreckovic@bristol.ac.uk > >0117-928-8606 > > > > > ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606 --- End Forwarded Message --- ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
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