Re: sorter counts.

From: Joseph Webster (J.Webster@centenary.usyd.edu.AU)
Date: Wed Dec 08 1999 - 22:20:30 EST


At 10:57 8/12/1999, Jill Martin wrote:
>      Hi Gang,
>           Just to get this in black and white, as most non sorting
>experts look at the numbers on the Vantage  and assume that is what they
>are going to get.    No one listens to me.     Should the end user be
>responsible for doing a cell count on the sorted cells?
>
>           Jill

YES !!!

I always point out to users that the sorter's count is approximate,
usually overestimated by something between 10% and 50% depending
on all those other factors previously mentioned.

I also ask them to do a cell count before sorting, sometimes it
happens....

      Joseph.

--
Joseph Webster
Flow Cytometry Facility
Centenary Institute



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