Hi Katja
Is that thank you line at the bottom your text? I wonder if all my email
messages are doomed to scramble.
Anyhow, thanks for the website reference which is usefull indeed.
Unfortunatelly the max. excitation wavelength is indeed misleeding as it
doesn't make you neccesarily aware of the problem of excitation-crosstalk.
Propidium Iodide might serve as an example as most of us excite it with the
488nm line of the argon LASER, despite it's optimum excitation in the green.
Thus I prefer Howards "excitation/emission -zone" plot.
The list has also triggered an additional question. Listed there is TrueRed, or
more precise PerCP-Cy5.5 Is Cy5.5 sufficiently different from Cy5 not to be
excited by the HENE as already indicated by Mario's message? If so, what do we
have to wait for, patents, licences or manufacturing problems?
Thanks
Gerhard
-----Original Message-----
From: Katja Adolf [SMTP:kad@mailme.dk]
Sent: Friday, December 03, 1999 2:27 PM
To: Cytometry Mailing List
Subject: Re:4 colours on FACS Calibur- UPDATE
----- Original Message -----
From: Gerhard Nebe-von-Caron <Gerhard.Nebe-von-Caron@Unilever.com>
To: cyto-inbox
Sent: 2. december 1999 10:19
Subject: RE: Re: 4 colours on FACS Calibur
>
>Hi Mario
>
>Also, would you be in a position to make up a table of names the companies use
>for the various base conjugates, their emission / excitation properties. Or has
>howard perhaps already one in preparation similar to the one in his book with
>the various dyes and the major lines from the available sources?
>
Hi Gerhard,
there is such a list about fluorochromes, but I dont know how complete it is,
at:
http://flowcyt.salk.edu/fcm/fluo.html
Best regards, Katja
**************************************************
Katja Adolf, PhD student
Aarhus University Hospital
Allergy and Lung Research Clinic
Nørrebrogade 44, DK-8000 Aarhus C, Denmark
Phone +45/ 8949 2106 Fax +45/ 8949 2110
email: kad@mailme.dk Also, I
wanted to REALLY THANK all who replied to the 4 colours on
Calibur thread. We were overwhelmed by the number of really qualified
ideas! We had a BD technician adjusting the machine (unfortunately only
to assure beads/ FACS Comp performance and not our cells) and go ahead
now with testing PE-Cy7 clones performance in our panel and readjusting
the compensations for the different PE-Cy5 clones, if possible.
**************************************************
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:18 EST