Here we routinely use polystyrene round bottom plates, for the following
reasons:
1. Less chance of compaction of cells in bottom of V
2. Both the granulocytes and platelets are only used in serological studies.
For any of my work involving platelets in a resting state I use
polypropylene. Although I've found that I lose more platelets in a PP 96
well plate. This is probably due to the less aggressive centrifugation
though.
Craig Turner
IBGRL
----------
From: robertar@UMDNJ.EDU
To: cyto-inbox
Subject: Re: Staining in 96 well plates: TC treat
Date: Saturday, December 04, 1999 02:14
I routinely use polystyrene Vee-bottom wells for my staining because I
believe I lose fewer cells than with U-bottom wells. I wonder if V-bottom
wells in polypropylene would be even better? Any opinions on this? Are
these plates available?
Art
On Thu, 2 Dec 1999, Larry Arnold wrote:
>
> John
>
> It's not a dumb question.
> We prefer round bottom polyPROPYLENE. Better for the cells.
>
> Larry Arnold
>
> At 08:54 PM 12/1/99 -0500, John D. Altman wrote:
> >
> >This is a dumb question.
> >
> >We stain cells in 96 well round bottom polystyrene plates. Does anyone
know
> >if it matters whether we use TC treated plates compared to non-treated
> >plates?
> >
>
> Larry W. Arnold, Ph.D.
> Res. Assoc. Prof.
> Director, Flow Cytometry Facility
> Department of Microbiology and Immunology
> CB# 7290
> University of North Carolina
> Chapel Hill, NC 27599
> Phone: 919-966-1530
> FAX: 919-962-8103
>
__________________________________
Arthur Roberts
Dept. of Medicine
Robert Wood Johnson Medical School
email: robertar@umdnj.edu
phone: 732-235-7790
Fax: 732-235-7792
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