I have had excellent results with the polyclonal Goat anti-mouse abs from both Southern Biotec and Caltag when used in this manner. Caltag offers more choices of flurochrome. As one might expect, polyclonal secondary Abs work quite well on fixed Ig stained cells; even if one epitope is destroyed by fixation, others are left intact. Do a careful titration of the Ab to optimize S/N. Calman > ---------- > From: Fang-Yao (Stephen) Hou > Sent: Friday, December 3, 1999 3:59 PM > To: Cytometry Mailing List > Subject: looking for 2nd step reagents good for fixed murine moAB > > > Dear cytometry users: > > One of my coworkers asks me to search for a good 2nd step antiboy which > will > bind to fixed murine monoclonol antibodies (IgG2b) specific for human CD > antigens. I called several vendors and they do not have data demonstrating > the binding of their 2nd step reagents on cells after staining and fixing. > They can only advise me to try... I appreciate any one here who has tried > this before, or can point us a direction to look for. Thanks. > > Sincerely, > Fang-Yao Stephen Hou > >
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