A number of investigators here are using virus that contain a gfp gene to
evaluate infection. At least one other cojugates his virus with fluorescein,
but I don't know how he accomplishes this.
David McFarland
Howard Hughes Medical Institute
Flow Cytometry Facility
Vanderbilt University Medical Center
---------------------- Forwarded by David McFarland/VUMC/Vanderbilt on 12/01/99
05:08 PM ---------------------------
"Nadeem Sheikh" <nadeem@bart.rprc.washington.edu> on 11/30/99 07:45:33 PM
(Embedded image moved to file: pic18232.pcx)From:(Embedded image moved to
file: pic04198.pcx)"Nadeem Sheikh" <nadeem@bart.rprc.washington.edu> on
11/30/99 07:45 PM
To: Cytometry Mailing List
<cytometry@flowcyt.cyto.purdue.edu>
cc: (bcc: David McFarland/VUMC/Vanderbilt)
Subject: detecting vaccinia infection
We would like to be able to detect and quantify vaccinia infection of target
cells (transformed BLCLs) - are there any "vaccinia" antibodies/protocols
available to do such a thing ? Any advice would be helpful. Thanks !
Nadeem Sheikh,
****************************************************************************************
RPRC Department of
Pharmaceutics
University of Washington University of Washington
Box 357331 Box 357610
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e-mail : nadeem@bart.rprc.washington.edu
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