Dear flowers, after having stained a phytoplankton sample with the green DNA stain Sytox-Green, we saw something quite unexpected: in addition to our expected G1 and G2/M peaks, we found 2 more green fluorescing peaks. It is definitely a unialgal culture. The peaks have the same heights and widths, but different areas. Given the geometry of a signal peak, how can two peaks have identical heights AND widths, but different areas?? Please refer to the attached powerpoint file, which contains the plots. Any clues are welcome! Marcus, Erik and Uwe ___________________________________________________________ Marcus Reckermann Forschungs- und Technologiezentrum Westküste der Universität Kiel (FTZ) (Research and Technology Centre Westcoast of Kiel University) Hafentoern D-25761 Buesum Tel. +49 (0)4834-604-204 or -261 Fax. +49 (0)4834-604-299 E-Mail: recker@ftz-west.uni-kiel.de FTZ Homepage: www.uni-kiel.de/ftzwest/ Flow Cytometry Homepage: www.uni-kiel.de/ftzwest/ag1/flow/flow-home-d.htm (deutsch) www.uni-kiel.de/ftzwest/ag1/flow/flow-home-e.htm (english) ________________________________________________ Keep your computer busy with www.setiathome.ssl.berkeley.edu
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