HELP!!! UNIDENTIFIED CLUSTERS!!! - Attachment!!

From: Marcus Reckermann (recker@ftz-west.uni-kiel.de)
Date: Thu Nov 25 1999 - 12:47:35 EST


Dear flowers,

after having stained a phytoplankton sample with the green DNA stain
Sytox-Green, we saw something quite unexpected: in addition to our expected
G1 and G2/M peaks, we found 2 more green fluorescing peaks. It is definitely
a unialgal culture. The peaks have the same heights and widths, but
different areas. Given the geometry of a signal peak, how can two peaks have
identical heights AND widths, but different areas?? Please refer to the
attached powerpoint file, which contains the plots. Any clues are welcome!

Marcus, Erik and Uwe

___________________________________________________________
Marcus Reckermann
Forschungs- und Technologiezentrum Westküste der Universität Kiel (FTZ)
(Research and Technology Centre Westcoast of Kiel University)
Hafentoern
D-25761 Buesum
Tel. +49 (0)4834-604-204 or -261
Fax. +49 (0)4834-604-299
E-Mail: recker@ftz-west.uni-kiel.de
FTZ Homepage: www.uni-kiel.de/ftzwest/
Flow Cytometry Homepage:
www.uni-kiel.de/ftzwest/ag1/flow/flow-home-d.htm (deutsch)
www.uni-kiel.de/ftzwest/ag1/flow/flow-home-e.htm (english)
________________________________________________
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