Fixation of Reticulocytes

From: Dagna Sheerar (dagnas@primate.wisc.edu)
Date: Wed Nov 24 1999 - 12:27:29 EST


Hi Flowers:

Does anyone out there have a protocol for fixing reticulocytes for flow
cytometry?  We'll be staining for the RNA in the retics, so we're looking
for something that will allow the dye (Corophosphine -O) to get into the
cell and interact with the RNA.

Thank you in advance!

Dagna Sheerar
Wisconsin Regional Primate Research Center
1220 Capitol Court
Bldg. #2  Room #215
Madison, Wi 53715
608 265 3796
dagnas@primate.wisc.edu



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