Hi! You can find our protocol for acute leukemias on http://www.ki.se/onkpat/res_area/patologi/sff/leukly.html For follow-up studies we use individual protocols that depend on the immunophenotype from diagnosis. Using "live gate" analysis one can get sensitivity of detection 10-3 - 10-4. Some references: Cytometry 1999 Aug 15;38(4):139-52 J Clin Oncol 1998 Dec;16(12):3774-81 Leukemia 1999 Mar;13(3):419-27 Clin Chem 1999 Oct;45(10):1708-17 Blood 1997 Sep 15;90(6):2465-70 best wishes Anna Anna Porwit Heamatopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
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