Dear Sarah:
Intraprep works great on our leukemia cells which don't like too
much manipulation. As far as the change in scatter that you see, I must
assume you are trying to compare the scatter with unfixed and unpermed
cells. it is normal to see a change in scatter when you permeabilize and
stain cells intracellularly. We just find that there is less of a change
between untreated and fixed/permed cells when we use Intraprep. Some of
the other products on the market put the fix and perm solutions together
and that is rather harsh on some cell types. But the Intraprep is more
gentle (on our cells at least). its a good product (and easy to make
yourself too).
Dr. Susan J. Zunino
Lehrstuhl fur Genetik
University of Erlangen
Staudtstr. 5
91058 Erlangen
Germany
Tele. 49 9131 8528784
FAX 49 9131 8528526
e-mail: szunino@biologie.uni-erlangen.de
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