It falls in the FITC channel. Brian Newsom -----Original Message----- From: Maciej Simm [mailto:simmmmer@yahoo.com] Sent: Monday, November 15, 1999 2:39 PM To: cyto-inbox Subject: monos, monos, monos.... Hello everyone. First, a newbie dye-chemistry question: is the emission for Rhodamine 123 similar to PE or FITC or PerCP? Assuming the basic FACScalibur laser configuration ( I don't remember the wavelenghts). I tried to resolve monocytes using a perCP cd4 antibody. In "unstimulated" (as in background level of activation) PBMC's CD4 can resolve between lymphocytes and monocytes b/c of level of expression. I know there are more efficient ways of doing this 4ex. CD14 and CD33. But in this experiment (oxidative burst assay) I was not able to detect the FL3 signal at all! It was suggested thereafter that CD4 gets dropped during activation and that could be my problem. Has anyone tried adding a PerCP marker to the burst test to enhance gating? I would apprieciate any suggestions. Maciej ===== `---------------------------------------------` | Maciej S. Simm | 525 E 68th Street | | Research Technician | Room N-805 | | Cornell Medical Center | Tel. 212.746.3428 | `---------------------------------------------` __________________________________________________ Do You Yahoo!? Bid and sell for free at http://auctions.yahoo.com
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