Dear flowers, I`d like to induce apoptosis in PBMCs using monoclonal antibodies against CD95 (clone DX2) and Protein G. I used freshly thawed PBMCs and IL2 activated LAK-cells but until now failed to get cells positively stained with Annexin-5-FITC. Even though I tried different antibody concentrations (5-20µg/ml) and different incubation periods (4-24h) no apoptosis was induced. I would be very grateful if anyone could help me. Marc Marc Jacobsen Klinische Neuroimmunologie MZ Nervenheilkunde - Neurologische Klinik mit Poliklinik Rudolf-Bultmann-Str.8, 35039 Marburg, Germany, Fax +49 6421 28 65480, Phone +49 6421 28 65480
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