I am currently caring for a patient diagnosed elsewhere with CLL clinically 5 years ago. His counts were stable for 1 1/2 yrs and then he was lost to followup. He was clinically stable until presenting two weeks ago with a febrile illness, lymphadenopathy and the following lab work: WBC 540K Hct 36 Plt 81 No evidence of DIC On peripheral smear he has both small lymphocytes (predominant), larger lymphoid appearing cells, and a significant number (5%) of monocytoid appearing cells with abundant somewhat granulated cytoplasm, folded nuclei, eccentric nuclei, rarely nucleolated. There are relatively rarer prolymphs. the bone marrow is similarly involved. The lymphoid cells mark as monoclonal B-cells CD5,19,22,23,25,DR+ lambda light chain positive (strong, not dim) the nonlymphoid, monocytoid cells stain CD4,7,10,33,13,14,15,DR+ and are CD34 negative. Could anyone comment on this monocytoid population and its implications for a coexistent myelodysplastic syndrome vs. M5 AML? Thank you. ---------- Forwarded message ---------- Date: Tue, 9 Nov 1999 17:23:40 +0000 From: Matthias Haury <mhaury@igc.gulbenkian.pt> To: cyto-inbox Subject: Partec vs Calibur.... Hello everybody, I know that this has been discussed a bit, but I would like to know from some Partec users their experience with the machines... especially regarding: - immunophenotyping - bacterial analysis - mycoplasms - software - compensation - sensitivity - sorting option I know the FACScalibur very well (and I am very happy with it..) but I have never really played with a Partec machine and have a friend who wanted to know the differences between a Calibur 3 Colors and a Partec/Dako PAS/Galaxy 3 Colors (one 488 laser). I have read the specs on the web, but I guess real world experience is worth much more than commercial arguments... therefore feel free to send me your comments, but it has to be fast, because they have to make a decision by the end of the week... thanks very much, Matthias _____________________________________ Dr. Matthias Haury Instituto Gulbenkian de Ciencia Unidade de Imagiologia Celular Rua Da Quinta Grande N° 6 - Apt.14 P-2781 Oeiras Codex Portugal Email: mhaury@igc.gulbenkian.pt Tel: + 351 21 440 79 85 Office Tel: + 351 21 440 79 10 Secretary Fax: + 351 21 440 79 70 _____________________________________
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