PE may have a higher quantum yield, but APC often gives better resolution in "real life". This could be due to better efficiency of conjugation or the signal to noise at the excitation/emission wavelengths used. Also,think about amplification using biotin-avidin. Or, sometimes a polyclonal goat anti-rat (if your mABs are rat) can be much better than biotin-avidin -we have had great luck with a reagent from Caltag. Or you can use anti-PE, and as did my colleagues: J Exp Med 1995 Jun 1;181(6):2029-36 Fas antigen stimulation induces marked apoptosis of T lymphocytes in human immunodeficiency virus-infected individuals. Katsikis PD, Wunderlich ES, Smith CA, Herzenberg LA, Herzenberg LA ======================================================= Rachel M. Gerstein, Ph.D. Department of Molecular Genetics and Microbiology Graduate Program in Immunology/Virology University of Massachusetts Medical School 55 Lake Avenue North Worcester, MA 01655-0002 (508) 856-1044 (508) 856-5920 (FAX)
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