Hi, all . . . I'm sorting sciatic nerve cells, specifically neural stem cells as a target. We find that the sort recovery (actual count vs. instrument count) is often less than 70%, perhaps as low as 50%. The optimal breakoff has been verified using 10 micron particles to be accurate, and we see much higher recoveries with other cell types. This has been done on a Vantage SE, either high-speed or at low pressures, using sort envelopes of 1, 1.5, 2, and 3 drops. Threshold rate is ~300-600 cells/sec, so we're not talking high aborts. I can only speculate that these cells are not traveling uniformly in the stream . . . Anyway, any ideas? -- Mark A. KuKuruga, Managing Director University of Michigan Core Flow Cytometry <http://www.cancer.med.umich.edu/flow_cytometry> phone: 734-647-3216 fax: 734-936-7376 kukuru@umich.edu
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