Dear all Sneza asked about using a Facs for absolute cell counts/concentration. You may wish to refer to the following article: THE QUANTITATION OF THE ABSORPTION OF MICROPARTICLES INTO THE INTESTINAL LYMPH OF WISTAR RATS AU: JENKINS_PG, HOWARD_KA, BLACKHALL_NW, THOMAS_NW, DAVIS_SS, OHAGAN_DT JN: INTERNATIONAL JOURNAL OF PHARMACEUTICS, 1994, Vol.102, No.1- 3, pp.261-266 Using an EPICS V I found that for a narrow dynamic range, i.e. 1-1000, I could use an internal standard to calculate the concentration. For a much broader range, I found it necessary to replace the sample input system with a syringe pump at a constant velocity and then just count the number of particles per unit time. Regards Nigel Blackhall, Experimental Officer, Plant Science Division, School of Biological Sciences, University of Nottingham, University Park, Nottingham NG7 2RD, UK Tel +44 115 9515151 ext 18501 Fax +44 115 9513298
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