For ongoing flow cytometry-based studies we need to prepare single
cell suspensions from large numbers of mouse spleens, typically
100-150 or more spleens, pooled in groups of 5. We do this now by
mechanical disruption (frosted microscope slides or stainless steel
mesh screens and the like). The procedure is, needless to say, tedious
and time consuming. Can anyone suggest better methods or does anyone
know of devices that would do the mechanical disruptions on multiple
samples (20 - 30) at once?
Gib Otten
Chiron Corp
Emveryville, CA
email to: Gillis_Otten@cc.chiron.com
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