I posted a question about tunel a few days ago. I was asking about the specificity of dctp (deoxy cytosine triphosphate) for apoptosis. According to the responses I received, it is specific for strand breaks that occur enzymatically during apoptosis and no dead cell discriminator is needed as it is when using AnnexinV. Due to my poorly worded question some people thought I was asking if PI was specific for apoptosis. I know that this isn't the case. Thanks for clearing that up for me. David McFarland Howard Hughes Medical Institute Flow Cytometry Facility Vanderbilt University Medical Center
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