Hi, 1. Neutrophils may have lower SSC if they are hypogranular - that can be checked on the morphology of PB smear. Moreover if the analysis of the sample was delayed neutrophils can degranulate. We have observed unspecific binding of CD61 to neutrophils (and myeloid precursors) in BM samples even after a couple of hours. Therefore if M7 is suspected we always confirm the staining with immunohistochemistry (APAAP). 2.The population of B cells you describe are the normal B cell precursors (see Lucio et al., Leukemia, 1999,13,419-427) - except for the dimMPO. Are you sure it was specific? Best wishes Anna Anna Porwit Heamatopathology Lab. Department of Pathology Karolinska Hospital, Stockholm anpo@mb.ks.se tel.:+46-851774518 fax.:+46-851775843
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:54:08 EST