Dear flowers! We got interested in natural killer-T (NK-T) cells and we would like to ennumerate them in peripheral blood. Which antibodies should we use? As far as I can see they co-express TCR alpha beta and TCR gamma delta. Do they stain with CD56 like NK cells? Do you know the expression pattern of CD4 and CD8? Thanks for help, Robert Gniadecki, MD Dermatology University of Copenhagen, Denmark ______________________________________________________ Get Your Private, Free Email at http://www.hotmail.com ROBERT NK cells also express zeta chains (but intracellulary). Indirectly you can ennumerate with anti CD3zeta, I normally use CD3FITC/CD16+56PE from Becton Dickinson, and de porcentage and absolute number correlate well with CD3(-)/zeta(the cells. JUAN LUIS CASTILLO N. CITOMETRIA DE FLUJO HOSPITAL DEL TRABAJADOR CONCEPCION CHILE PHONE: 56-41-201722 FAX: 56-41-311008 EMAIL: axelyoyi@entelchile.net
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