Hi, One of our clients asked me the effect of lower viability on the Vario MACS separation purity. Since antibodies nonspecifically bind to dead cells, is this something to worry about in say a population of cells that has an 80% live count after thawing? Is it necessary to run a ficoll gradient to enrich for the live cells before MACS purifying? What is an acceptable live count before proceeding? I would like to hear from anyone with experience along these lines. Thanks, Kristi R. Harkins, PhD Director, Cell & Hybridoma Facility Iowa State University 1104 Molecular Biology Building Ames, IA 50011 Office (515) 294-2472 Lab (515) 294-8504 FAX (515) 294-0453 http://www.biotech.iastate.edu/facilities/cellhyb/homepage.htm
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