RE: Human vs mice markers for cell lines in culture

From: Gerstein, Rachel (Rachel.Gerstein@umassmed.edu)
Date: Sun Oct 17 1999 - 12:28:57 EST


If you stain with antibodies specific for murine class I MHC and for human
class I MHC, you should be able to identify human, mouse or hybrid cells.
=======================================================
Rachel M. Gerstein, Ph.D.
Department of Molecular Genetics and Microbiology
Graduate Program in Immunology/Virology
University of Massachusetts Medical School
55 Lake Avenue North
Worcester, MA 01655-0002
(508) 856-1044
(508) 856-5920 (FAX)


> ----------
> From: 	Carmen Raventos-Suarez
> Sent: 	Thursday, October 14, 1999 3:29 PM
> To: 	Cytometry Mailing List
> Subject: 	Human vs mice markers for cell lines in culture
> 
> <<File: carmen.raventossuarez.vcf>>
> Hello everybody:
> Let me take advantage of this wonderful resource for information
> by asking:
> 
> What is the status on the availability of differential markers to
> characterize human tumor cell lines established in culture from
> tumor xenographs growing in mice?
> I mean, What is the most reliable answer to establish if they are
> human or mice or an hybrid?
> 
> This is a an new challenge for me and I wonder if anyone can give
> me a hand in making some decisions.
> Thank you
> Carmen Raventos-Suarez
> BMS
> E-mail: carmen.raventossuarez@bms.com
> 
> 



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