I am looking for a protocol for the isolation of mouse hepatocytes for immediate use on flow. I have found several old protocols isolating rat hepatocytes, but the majority of these entail growing the hepatocytes in culture or fixing in ethanol for future use. I am looking to analyze by flow immediately with Annexin V and PI and none of these protocols seem to be compatible. Thanks for any input. Lee Adamson Shriners Hospital for Children Northern California
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