Hello, I have an investigator who is atttempting to evaluate mitochondion membrane potential with JC1 after treatement with cytotoxic drugs. Under the fl microcope, we observe in the control cells, very few mitochiondia (orange) at all. Could this be a stain concentration effect ( 1uM)? Is there any practical experience with JC1 and HeLa cells we should be aware of....? The cells were trypsinized to create the suspension, is this being too harsh? Regards, Dave G. Dave Gebhard--Director FACS Facility Albert Einstein College of Medicine Chanin Building 309 1300 Morris Park Avenue Bronx, New York 10461 718-430-2724/ 3573 FAX 718-430-8550
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