Wow! I'm starting to feel a little inadequate here, but you guys are working a lot harder than I am. I have a .2 um in-line filter on my MoFlo, so I rinse the tank with a little ethanol at the beginning of the week, then fill up the tank daily in the open. We've never had a contamination yet, and we routinely swich back and forth between bacteria, yeast, and mammalian cells. I pay a lot more attention to the sample lines- I use ethanol, CoulterCleanse, and sterile H20 to set up for a sterile sort, but I don't ever pay much attention to the sheath tank. Carol --------------------------------------------------------------------------- Carol Oxford _| _| _|_|_|_| _|_|_| Manager, UC Davis Optical Biology Lab _| _| _| _| _| Tupper Hall, rm 3439 _| _| _| _| _| University of California _| _| _| _| _| Davis, California 95616 _| _| _| _| _| (530) 752-7205 _| _| _| _| _| (530) 752-4548 fax _|_|_|_| _|_|_|_| _|_|_| (530) 523-0967 pager cloxford@ucdavis.edu University of California, Davis ---------------------------------------------------------------------------
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