Synovial tissue replies

From: Dash, Laura M (lmd2335@GlaxoWellcome.co.uk)
Date: Mon Oct 04 1999 - 09:10:01 EST


Thanks Tanja and Craig for your suggestions. 
I'll pass them onto my colleagues and let everyone on the list know
what happens. Tanja suggested removing adherent cells and although the
adherent cells will be of interest, this could be useful for the initial
analysis of lymphocytes and monocytes. Craig suggested using NaVO4 to
block detachment induced death and EDTA in buffers to wash the cells.

It never ceases to impress me how helpful people are on this mailing list!

Laura.



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