>Hi Carl, > >I did a few experiments using FITC, PE, PE-Cy5 and PE-Cy7 >like 4-color analysis from one laser line (488nm) on a >FACSVantage. Compensation for overlaps between PE and its >conjugates is a bit tricky but it's not more then 30% >either way and you just need nice monocolor controls to set >it right. We are using Streptavidin-Pe-Cy7 which tends to >be a little sticky and careful titration would be >necessary. > >Regards, >Sasha > >On Mon, 27 Sep 1999 17:18:34 +0200 ><carl-magnus.hogerkorp@molmed.lu.se> wrote: > >> >> Dear All, >> >> does anyone have any experience in analyzing preparations stained with Ab's >> conjugated with PE, PE-Cy5 and PE-Cy7 at the same time. Are there any >> problems associated to this kind of stainings? >> >> >> Carl-Magnus H–gerkorp >> Stem cell laboratory >> Department of Internal Medicine >> University Hospital of Lund >> Sweden >> >> > >---------------------- >Dr Sasha Sreckovic >Dept Path & Micro >University of Bristol >University Walk >Bristol, BS8 1TD, UK >Sasa.Sreckovic@bristol.ac.uk >0117-928-8606 Hi all, I haven't used the PE-Cy7 conjugate, but have used the APC-Cy7 (PharRed) conjugate. I found the PharRed dye congugate to decouple. This was evident in single stain test controls where I saw a population in the APC channel when running PharRed. Has anyone else see this? How do your single test controls look with the PE version? Andy. Andy Riddell PNAC Division MRC LMB Hills Road Cambridge UK tel: (0)1223 402218 fax: (0)1223 412178 email ar3@mrc-lmb.cam.ac.uk
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