Hi Carl, I did a few experiments using FITC, PE, PE-Cy5 and PE-Cy7 like 4-color analysis from one laser line (488nm) on a FACSVantage. Compensation for overlaps between PE and its conjugates is a bit tricky but it's not more then 30% either way and you just need nice monocolor controls to set it right. We are using Streptavidin-Pe-Cy7 which tends to be a little sticky and careful titration would be necessary. Regards, Sasha On Mon, 27 Sep 1999 17:18:34 +0200 <carl-magnus.hogerkorp@molmed.lu.se> wrote: > > Dear All, > > does anyone have any experience in analyzing preparations stained with Ab's > conjugated with PE, PE-Cy5 and PE-Cy7 at the same time. Are there any > problems associated to this kind of stainings? > > > Carl-Magnus Högerkorp > Stem cell laboratory > Department of Internal Medicine > University Hospital of Lund > Sweden > > ---------------------- Dr Sasha Sreckovic Dept Path & Micro University of Bristol University Walk Bristol, BS8 1TD, UK Sasa.Sreckovic@bristol.ac.uk 0117-928-8606
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