I have aquestion concerning viability measurement of cardiac myocytes (mouse). In our test model we need to know the cell viablity rate under several defined conditions. We tried propidium iodide unsuccessfully as there was no staining that could be correlated to necrotic myocytes. Can this be due to the high autofluorescence of cardiac myocytes? Another fact seems to be that myocytes are susceptible to mechanical stress occuring during analysis. Can anyone help with these problems? Matthias Heil ______________________________________________________________________ Dr. Matthias Heil, Dipl.Biol. Voice: ++49 (0) 6032 705-414 Max-Planck-Institut Fax: ++49 (0) 6032 705-419 Exp. Kardiologie§ E-mail: m.heil@kerckhoff.mpg.de Benekestr. 2 | D-61231 Bad Nauheim | Germany |
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