help on apoaequorin

From: zhangj (zhangj@JingXian.xmu.edu.cn)
Date: Wed Sep 22 1999 - 00:54:15 EST

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Hi all,
We have cloned the apoaequorin gene from a kind of jellyfish and expressed it in E.coli, now I want to detect its bioluminescent activity, but I don't know if I can use firefly luciferin instead of coelenterate luciferin, because I cannot find any commercial resources for the coelenterate luciferin. Does anyone have some suggestion about it? Any advice would be very appreciated.

Thanks in Advance

Zhang,Jun
The State Lab for Tumor Cell Engineering
Xiamen University
Xiamen,Fujian,361005
P.R.China

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