Dear everyone out there in flow land. I have a researcher who is trying to detect MDM2 by flow cytometry. So far we have tried different methods for permeabilisation and fixation with nio success. We know that the antibody is working because it is positive in cytospins with acetone fixation in the CEM and U20S cell lines, but we are useccessful to detect any signals by flow. Help Please!! Is anyone out there doing it by flow who is willing to discuss what is it that we are doing wrong, or who could help with ideas or references. Thank you in advance. Ricardo ========================================================== Mr. Ricardo Morilla Academic Department of Haematology & Cytogenetics The Royal Marsden Hospital 203 Fulham Road, London SW3 6JJ, UK Tel +44 (0) 20 7808 2882; Fax +44 (0) 20 7351 6420 ==========================================================
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