Hi flowers, concerning the discussion about TO-PRO-3 I just want to mention, that I got a paper accepted about the use of TO-PRO-3 instead HOECHST dye for the detection of BrdUrd together with PI for total DNA measurement. It is about as good as the old BrdUrd Hoechst quenching technique, but does not require a UV laser. May be that is of interest for you all. With best regards from munich Wolfgang Beisker Dr. Wolfgang Beisker GSF - National Research Center for Environment and Health Flow Cytometry Group Ingolstaedter Landstr. 1, 85764 Neuherberg, Germany
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