Hi everybody, I´d like to know if anyone here have any experience with surface immunophenotype and cell cycle. I have PI, RNAse and FITC-conjugated antibodies...but I don`t know the best protocol for permeabilization with ethanol !!! I would be grateful for any answer... Thank´s _________________________________________ Zilton Vasconcelos Laboratory of Immunology Bone Marrow Transplantation Unit (CEMO) Brazilian National Cancer Institute (INCA) Praca Cruz Vermelha, 23 - 6 andar Centro, Rio de Janeiro, RJ, Brasil 20230-130 Tel.: (55-21) 506-6677 FAX: (55-21) 509-2121 E-mail: zfmv@uol.com.br
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