I'm having a problem setting up an experiment for a researcher
on our Elite so I thought I'd go to the experts for help! The expt.
involves fusion between bull sperm and liposomes containing R18 or
fluorescein. I adjust the voltage in pmt 2 for fluoresein to get a
nice peak but have to turn down the voltage in pmt 4 to keep it in
the background . When I run R18 at those settings, the pmt 4 peak
is too low. If I adjust the voltages to get optimal peaks for each
dye separately no amount of compensation helps. Both pmt's are in
the log scale and the peak are by no means tight. Any suggestions?
Thanks,
Jan Brazolot
Plant Agriculture Dept.
University of Guelph,
Guelph, ON
Canada
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