Re: FLAG antibody

From: Rizwan Haq (haqr@oci.utoronto.ca)
Date: Sat Sep 11 1999 - 13:46:09 EST


As far as I am aware, ATCC sells the hybridoma that makes the related
FLAG antibody FLAG-M1. This is a calcium-dependent monoclonal that
recognizes the same epiptope as the M2 (and the M5). The M1 hybridoma
is called 4E11.

Rizwan Haq
Ontario Cancer Institute
Toronto, Ontario
haqr@oci.utoronto.ca


On Fri, 10 Sep 1999, Keri Tate wrote:

> 
> Hello all,
> 
> I am looking for an antibody called "FLAG-M2".  It is a non calcium
> dependent  IgG monoclonal antibody specific for an enterokinase
> sequence.  This antibody is often used in protein expression systems to
> cleave off the tag sequence (enterokinase) after protein purification.
> I know that Sigma/Kodac sells this antibody (or one similar to it) but I
> was also told that it is available from ATCC (much cheaper, of course).
> If anyone knows the history of this antibody or has a reference, please
> contact me.
> 
> Thank you
> Keri Tate
> (408) 585-3907
> keri.tate@arborvita.com
> 
> 



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