Re: LDS-751

From: Howard Shapiro (hms@shapirolab.com)
Date: Thu Sep 09 1999 - 17:49:05 EST


Jose Stoute writes:

>Does anyone know the address of a company in the US or Europe where I
>can purchase LDS-751? I need address, telephone and fax numbers that are
>not toll free. If there is a web site, that too would be helpful.
>Any help will be greatly appreciated.
>Thank you,
>--

The LDS-751 originally used by Loken, Terstappen, et al came from Exciton,
in Dayton, Ohio - they have a rudimentary web site, www.exciton.com, which
gives their phone numbers and e-mail addresses but does not list products.
Fisher Scientific (www.fishersci.com) sells LDS-751 at 10 mg for $68.00;
it's in their Acros Organics catalogue.  However, I have been told that
there are at least two different dyes being sold as LDS-751; one of them
works as a vital (but not stoichiometric) nucleic acid stain, as originally
reported, and the other is said not to.  I've used Exciton dye successfully,
but I bought it years ago.

-Howard


And by the way, malaria parasites stain very nicely with red-excited nucleic
acid dyes such as Oxazine 750, and could potentially be detected in a very
inexpensive flow cytometer with a red diode laser source - easily taken into
the field - if you're interested.



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