Dear ALL, WE would like to analyse the prescence of the FAS ligand on the surface of lymphocytes post bone marrow transplantation. However, this does not appear to be as simple as it sounds. It appears that permeabilisation of the lymphocytes are needed. Does any one have a reference I could use or any recommendations as far as permeabilisation solutions are concerned. Any advice would be most appreciated. Many thanks, Glenda Davison Dept. haematology Groote Schuur Hospital University of Cape Town Cape Town South Africa
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