Dear colleagues in FLOW: we are using the Vindelov method in a Facscan with Cellfit software to perform DNA ploidy. Lately, (coincidence, I hope)we have recorded wide CVs on deparaffinized breast Ca. cell suspensions. Our CONTROLS are always fine, either PB lymphs., fresh or deparaffinized tonsil materials. We would appreciate any suggestions on improving the CV on deparaffinized cancer tissues by FLOW. Could the incubation with some antibody (ie p53 if the propositus nuclei are positive by immunohisto/cytology), prior to passing the aliquot by the flowcytometer, (could) discriminate positive cancer nuclei from negative benign nuclei??. If such or similar discrimination is possible by gating (I don't have a sorter), would that yield or show that, perhaps, the CV is wide because there is a hidden SECOND peak on it??. Thank you in advance for any thought or responses. Victor A. Saldivar, MD <victor_saldivar@srhc.iwhs.org>
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