This may not be exactly what you want but it is better than being run over
by a train.............
In our laboratory do quite alot of T cell analysis using Immunoscope and
other fairly advanced technologies. However, to follow very small numbers
of cells we use a T Cell Clonality assay kit developed and sold by IVS
Technologies, a company located in Carlsbad, California (northern San Diego
County) with laboratories here in San Diego. The company makes a variety of
molecular diagnostic products including B cell and T cell clonality assay
kits, translocation testing kits (bcr-abl: t(9;22); bcl2: t(14;18); APL:
t(15;17)] and other reagents related to hematologic testing. They also do
inhouse testing and have an online internet ordering system at:
www.invivoscribe.com You can also order Toll free at (877) 431-6705.
Their assay kits use fluorescent PCR primer panels coupled with a capillary
electrophoresis or gene sequencing instrument to detect T cell populations
down to approximately 0.5%. It is a very rapid and inexpensive technology.
I understand that they have made custom kits for specific applications and
specific subset testing for several of their customers. They also post test
results directly on a secure internet site for their clients, which reduces
turnaround time dramatically without compromising patient confidentiality.
A recent reference is Miller, et al., June 1999. Molecular Diagnosis
4(2):101-117.
Good luck.
Chris
--------------------------------------------------------------------------------
We are getting increasing numbers of requests from physicians asking us to
perform T cell subset testing on transplant patients who are receiving
immunosuppressive therapy. These specimens typically have <5% lymphocytes,
very low numbers of T cells and are very problematic when we use our
standard protocol. Can anyone give me any input as to how you handle these
specimens in your labs?
Chris Lena
Laboratory Technician
La Jolla Institue For Allergy and Immunology
phone:(619)678-4536
fax:(619)678-4595
cali@liai.org
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