Dear Lewis and Sebastian and people involved in this discussion You can also try to find the others GPI linked molecules defect on white blood cells. For example using a double staining with CD15 and CD16 on neutrophils, CD64 and CD14 on monocytes. CD15 and CD64 molecules are not GPI linked and so, can be used to gate respectively the PMN and monocytes cell populations. It is now very easy to see if the CD16 and CD14 are or not, expressed in cells of your PNH patient. Take care that some monoclonal antibodies against the CD16 molecule, recognise a molecule not GPI linked. This is a very simple alternative to CD55 and CD59 quantification on red blood cells, for example, following a red blood cells transfusion. Success Jean-Pierre Delville lewis lipsey (Medicine) wrote: > I would be interested in receiving your responses. I am following a > patient now with ?PNH but with equivocal results on flow for CD59. What is > the sensitivity/specificity of the finding? > > On Wed, 18 Aug 1999, Sebastian, J, Gelderbloem, Mr wrote: > > > > > Daer Flowers > > > > I have recently started using flow for test for PNH. We are using > > CD55 and CD59 and looking at granulocytes and erythrocytes. > > I would like to hear your experiences with this test. I also have a > > problem identifying the different types of PNH. At the moment we are > > only set up to identify PNH abnormalities. > > Please help. > > > > Yours sincerely > > Sebastian Gelderbloem > > > > -- Jean-Pierre DELVILLE MS ULB-Hopital Erasme Laboratoire d'hematologie Route de Lennik,808 1070 Brussels BELGIUM Tel 32 2 555 39 48 Fax 32 2 555 44 99 E-mail jdelvill@ulb.ac.be
This archive was generated by hypermail 2b29 : Wed Apr 03 2002 - 11:53:52 EST