Does monensin interfer with antigen presentation? I am planning studies on intracellular cytokin analysis after antigen specific stimulation of PBMC or whole blood. We use whole antigen protein, thus, the antigen must be processed before presenting to lymphocytes. Monensin is said to be non-toxic if used for less than 20 hours. 20 hours might be enough for antigen processing and presentation. Can we add monensin simultaneously with antigen, and incubate for 20 hours? Thanks much! Unni Haddeland, ph.D. Research Laboratory University College of Akershus Ringstabekkvn 105 1365 Bekkestua NORWAY
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