Hi! I am a new user of the FACS Calibur. I am looking into using flow to replace the 51Cr release CTL assay. Has anyone used the LIVE/DEAD Cell-Mediated Cytotoxicity Kit (L-7010) from Molecular Probes. I am not sure if the green flourescent label for the membrane, DIOC18 (3,3'-dioctadecyoloxacarbocyanine) which has a emission maximum of 501 nm will be detected optimally (excitation wavelength if 484nm) on the FACS calibur. If not, does anyone has a suggestion or know of a kit that will allow me to detect lysis of target cell versus effector cells. Thank you so much for your help and I look forward to your suggestions and recommendations. Sincerely, Mee H. Hoe,, Ph.D. Mojave Therapeutics, Inc.
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